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1. Staphylococcus epidermidis, a common cause of healthcare-associated infections, forms biofilms on medical devices. The production of polysaccharide intercellular adhesin (PIA) is a key factor in biofilm maturation.
The expression of PIA is primarily regulated by:
A) Horizontal gene transfer of the ica operon.
B) The stringent response pathway activated during nutrient limitation.
C) A two-component regulatory system involving AgrA and AgrC.
D) The icaADBC operon, whose expression is influenced by environmental cues.
2. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:
A) Giardia lamblia
B) Cyclospora cayetanensis
C) Cryptosporidium parvum
D) Entamoeba histolytica
3. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:
A) Inoculation with a bacterial suspension that is too dense
B) Use of expired antibiotic disks
C) Incubation at an incorrect temperature
D) All of the above
4. A research team is investigating the mechanisms of antifungal resistance in Candida albicans. They identify a strain that exhibits resistance to azole antifungal agents. Further analysis reveals overexpression of a specific ATP-binding cassette (ABC) transporter protein in the resistant strain. This mechanism of resistance involves:
A) Modification of the fungal cell wall, preventing azole penetration.
B) Alteration of the fungal ergosterol biosynthesis pathway, the target of azoles.
C) Increased degradation of the azole antifungal agent within the fungal cell.
D) Active efflux of the azole antifungal agent out of the fungal cell.
5. A patient with a history of intravenous drug use develops a fever and new heart murmur. Blood cultures are positive for Gram-positive cocci that are catalase-positive and coagulase-positive. The MOST likely complication of this infection is:
A) Glomerulonephritis
B) Embolic stroke
C) Septic arthritis
D) Reactive arthritis
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: A | Question # 3 Answer: D | Question # 4 Answer: D | Question # 5 Answer: B |
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